Weed Killer Radically Alters Frog Reproduction

Some “chemically castrated” males under observation developed into egg-laying females.

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By Janet Raloff, for Science News' Science & the Public Blog

Several months back, a Berkeley undergraduate began witnessing distinctly odd behavior in frogs she was caring for in the lab. At about 18-months old, some frisky guys began regularly mounting tank mates, as if to copulate. Except that their chosen partner was invariably male. He had to be. Because genetically, every animal in the tank was male.

What Ngoc Mai Nguyen didn’t know at the time was that something in the water had reproductively altered several of the frogs to the point that they not only became submissive, like females, but also apparently sent out come-hither signals to the bro’s in their tank. 

“We were conducting a double-blind study, so I didn’t know what treatment [if any] they were getting,” Nguyen recalls. She did know, however, that each animal had been bred to ensure it was genetically male.

So she informed her boss, biologist Tyrone Hayes. “I told Tyrone, ‘I don’t know what’s going on, but I don’t think this is normal.’” He asked her to log the guys’ behavior on a daily basis. And this confirmed some frogs definitely behaved like gals. Those who did even looked like females.

These frogs had been raised from hatching in tanks of water containing low levels of atrazine, a widely used weed killer. “What Mai discovered,” Hayes says, “is that when they’re in these tanks, atrazine-exposed males will copulate with other males.” Indeed, he notes, among the atrazine-exposed animals that Mai was caring for, some 30 percent exhibited this girly behavior. It’s something Hayes previewed last Tuesday (Feb. 23) in a briefing before the Illinois legislature, in Springfield — and will describe in a paper that his group plans to submit for publication later this week.

In some regions of the country, Hayes says, year-round atrazine pollution of surface waters downstream of crop fields can match the concentrations that he used — 2.5 parts per billion in water. The Environmental Protection Agency allows up to 3 ppb of the herbicide in drinking water.

The new observations, outrageous as they may sound, expand upon findings to be published online by Hayes’ team this week (ahead of print) in the Proceedings of the National Academy of Sciences. Those data demonstrate that chronic 2.5 ppb atrazine exposures can fully reassign the gender of some frogs. Indeed, the researchers report raising three generations of Xenopus laevis — African clawed frogs that serve as the amphibian equivalent of lab rats (from colonies that never contained a real female, one whose DNA contains the gene to develop into a mom).

Other research groups had seen evidence that atrazine can demasculinize — and in some instances feminize — exposed animals. But frogs don’t have readily visible sex chromosomes, so it was hard to tell sometimes if feminized males were actually masculinized females. Or vice versa.

To focus on effects in males only, Hayes' team reared animals, crossing them in ways to get a line of pure males. Genetic males, anyway. Then he raised some of them in clean water. Others grew from larval stage to three-years post metamorphosis in tanks laced with trace concentrations of atrazine.

And compared to males growing in clean water, those chronically exposed to the weed killer developed few sperm, produced very low testosterone levels and failed to “sing” the calls that should invite females or repel competing would-be suitors.

But some of the males didn’t just have diminished levels of the male sex hormone. Four out of 40 described in the new PNAS paper also produced elevated levels of estrogen, the primary female sex hormone (not as high as true females would develop, Hayes notes, but far higher concentrations than a male should ever have). These animals also developed external reproductive organs typical of females and exhibited female behaviors.

Two were opened up and their internal reproductive organs were characteristic of females as well. The remaining two transsexuals were introduced to males who had grown up in tanks of clean water. The would-be females accepted the advances of the males, allowing them to fertilize their eggs. Which grew into healthy frogs.