Inserted Gene Passed Along to Monkey Offspring

MALCOLM RITTER
AP Science Writer

NEW YORK—Scientists have shown that transgenic monkeys can pass a newly acquired gene along to their offspring, a "milestone" for creating animals with versions of human diseases for medical research.

While researchers have long created transgenic mice and other animals by giving them extra genetic material, monkeys offer a promising avenue for medical studies because of their similarity to humans.

Researchers have added genes to rhesus macaques before, but the new work with marmosets is the first to document that monkeys can pass an inserted gene along to future generations. That's important because it opens the door to creating colonies of such "transgenic" monkeys by breeding, which would be far simpler than the cumbersome process of making each animal from scratch by inserting genes into embryos.

The work is reported in Thursday's issue of the journal Nature by scientists at the Central Institute for Experimental Animals in Kawasaki, Japan, and elsewhere in that country.

The researchers plan to use transgenic marmosets to study such conditions as Parkinson's disease and Lou Gehrig's disease, or ALS, a statement from the institute said.

Anthony Chan of the Emory University School of Medicine in Atlanta said the result boosts his confidence that his transgenic macaques will also pass along their added genes to offspring, once they become old enough to reproduce.

For the study, the researchers used a gene that makes tissues glow under ultraviolet light, as an easy way to see where the gene is present. They put the gene in a virus that would insert it into the DNA of cells, and then injected the virus into marmoset embryos. From these embryos, five healthy marmosets were born. All showed evidence of having inherited the gene.

Later, one of those animals fathered a male by test-tube fertilization. The gene was shown to be active in the offspring's skin.

"The birth of this transgenic marmoset baby is undoubtedly a milestone," Gerald Schatten of the University of Pittsburgh School of Medicine and a colleague wrote in a Nature commentary.

Transgenic marmosets could be useful for studying infectious diseases, immunology and neurological disorders as well as some genetic disorders like muscular dystrophy, they wrote. But marmoset biology differs enough from humans to prevent study of other disorders like AIDS and tuberculosis, which can be approached instead through other monkeys that are more closely related to humans, they wrote.

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